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Bubbles and other variants |
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%20128.jpg) |
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In some Q-ball lines, a variety of curious bubble-like structures can be observed. Shown here are two time series from line 301b. The series at left shows a double bubble, a sphere within a sphere. The structure is situated at the end of a hypocotyl epidermal cell. Image frames were aquired approximately every two seconds, and are played back at 10 frames a second. |
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The time series at right was also aquired from hypocotyl epidermal cells. These "theta" structures appear as if two spheres have fused side by side with a planar partition between them. Punctate patches and strands of fluorescence can be seen inside the labeled shells of each structure. Same aquisition and playback parameters as the series at left. |
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The above stuctures resemble minimal energy configurations, such as are seen with soap films. One possibility is that these structures are artifactual distortions of a membrane compartment caused by over-expression of the fusion protein. |
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Tag 301b is an out of frame fusion to a non-Arabidopsis sequence, the tetR gene. This fusion was likely generated from vector sequences during construction of the library. |
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